12th Fetal Cell Workshop


Final program

Abstracts, list of participants and participating companies will be available since May 3, 2001.

Saturday May 12, 2001
Session A
(in main lecture hall)

FETAL CELLS IN MATERNAL BLOOD FOR NONINVASIVE PRENATAL DIAGNOSIS

Surname Name The title of abstract Country Min
Session A1: NEW MARKERS (chairpersons I. Van Wijk, I. Hromadníková) 9:30 - 10:10
Van Wijk Inge New potential markers for identification of trophoblast cell circulating in maternal peripheral blood. The Netherlands 10
Séveus Lahja Identification of fetal trophoblasts from peripheral blood using antibodies to intracellular antigens. An experimental study. Finland 10
Kilpivaara Outi Cytotrophoblast-specific antibodies for identification of fetal cells in maternal blood Finland 10
Vona G. Enrichment and identification of fetal cells circulating in maternal blood by the ISET and single cell DNA analysis - a non invasive tool for prenatal diagnosis France 10
Coffee break 10:10 - 10:25
Session A2: MOLECULAR CYTOGENETICS (chairpersons M. Hultén, K. Michalová) 10:25 - 11:10
Hromadníková Ilona Fetal cell separation and FISH analysis Czech Republic 10
Hulten May A novel approach for identification of foetal cells in maternal plasma UK 20
Martin Thomas Combined specific immunostraining of heamoglobin epsilon chain and FISH of nucleated red blood cells. Germany 15
Session A3: FETAL CELL CULTURE (chairpersons E. Guetta, M. R. de Alba) 11:10 - 12:05
Bohmer Ralph Experience with culturing fetal cells from maternal blood USA 15
O'Donoghue Keelin Selective expansion of fetal mesenchymal STEM cells over maternal cells for noninvasive prenatal diagnosis. UK 15
Hahn Sinuhe Inability to detect single fetal cells clones in fetal cell cultures from maternal blood Switzerland 10
Guetta Esther Fetal CD34+ cells in maternal blood: Feasible target cells for non-invasive prenatal diagnosis? Israel 15
Session A4: CLINICAL TRIALS 12:05 - 12:25
De Alba Marta Rodriguez Three year experience in the diagnosis on fetal cells from maternal blood Spain 20
Lunch
University Hospital Restaurant		 12:25 - 14:00
Session A5: TECHNICAL REPORTS (chairpersons H. Schuring-Bloom, T. Zolotukhina) 14:00 - 15:15
Shilova Nadezhda The detection of fetal nucleated red blood cells in maternal blood by flow sorting using intracytoplasmatic antifetal hemoglobin Russia 15
Martel-Petit Veronique A rapid and simple procedure using Kleinhauer test to detect fetal erythroblasts in the maternal circulation: preliminary results for prenatal diagnosis France 15
Shilova Nadezhda The comparative analysis of the efficiency of fetal cells isolation from maternal blood by different methods Russia 15
Schuring-Blom Heleen Analysis of fetal nucleated red blood and trophoblast cells on washings from CVS samples. The Netherlands 15
Zolotukhina Tatyana The use of fetal cells isolated from maternal blood by different methods for prenatal diagnosis Russia 15
Coffee break 15:15 - 15:30

Saturday May 12, 2001
Session B
(in main lecture hall)

FETOMATERNAL CELLULAR AND DNA TRAFFICKING

Surname Name The title of abstract Country Min
Charpersons: D. Bianchi, S. Hahn 15:30 - 17:20
Bianchi Diana Bi-directional cell trafficking and disease USA 30
Lo Dennis "Studies on bi-directional DNA trafficking" China/Hong Kong 30
Johnson Kirby FISH methods to detect fetal cell microchimerism in maternal tissue samples USA 20
Holzgreve Wolfgang Abnormal fetal cell trafficking in preeclampsia and other pregnancy related disorders Switzerland 30

Concert of chamber music - Kocian's Quartet:  A.Dvořák:
String Quartet in C major, KV 157
Allegro
Andante
Presto
W.A.Mozart:
 String Quartet in F major, op.96 "American"
Allegro ma non troppo
Lento
Molto vivace
Vivace ma non troppo		 18:00 - 19:00
Welcome party
University Hospital Restaurant		 19:00 - 22:00

Sunday May 13, 2001
Session C
 (in main lecture hall)

CLINICAL APPLICATIONS OF FETAL DNA IN MATERNAL PLASMA

Surname Name The title of abstract Country Min
Chairpersons: D. Lo, K. Klinger 8:30 - 10:20
Klinger Katherine Characterization and optimization of primer/probe sets for quantitation of fetal DNA in maternal plasma USA 20
Lo Dennis Clinical application of fetal DNA in maternal plasma China/Hong Kong 30
Hahn Sinuhe Prenatal diagnosis of fetal genes and pregnancy related disorders using fetal DNA Switzerland 20
Smid Maddalena Quantitative analysis of fetal DNA in maternal plasma in pregnancies affected by IDDM Italy 15
Bianchi Diana Thoughts on the origins of fetal DNA in the pregnant woman USA 25
Coffee break 10:20 - 10:35

Sunday May 13, 2001
Session D
 (in main lecture hall)

NEW MOLECULAR GENETIC AND CYTOGENTETIC METHODS FOR PREIMPLANTATION AND PRENATAL GENETIC DIAGNOSIS

Surname Name The title of abstract Country Min
Session D1: PRECONCEPTIONAL DIAGNOSIS (chairpersons: F.Pellestor, C. Petit) 10:35 - 11:20
Pellestor Franck Multicolor PRINS for chromosome 13, 16, 18, 21, X and Y on lymphocyte and human sperm preparations France 15
Petit Cristophe Screening of the first polar body for aneuploidy detection France 15
Tomi Diana Frequency of allele dropout in PCR analysis single cells Germany 15
PREIMPLANTATION DIAGNOSIS
Session D2: MOLECULAR CYTOGENETICS (chairpersons: Y. Verlinski, K. Johnson) 11:20 - 12:15
Brett Williams Detection of multiple chromosomes in single cells with peptide nucleic acid (PNA) probes USA 20
Verlinsky Yuri Full karyotyping and testing for translocations in single cells USA 20
Ogur Gonul Pre-clinical work-up of preimplantation genetic diagnosis for chromosomal translocation carriers Belgium 15
Session D3: MOLECULAR GENETICS – CLINICAL TRIALS (chairpersons: A. Kuliev, K. Sermon)
 12:15 - 13:20
Rechitsky Svetlana Avoiding misdiagnosis in single cell genetic testing for Mendelian disorders USA 15
Sermon Karen PGD diagnosis of monogenic disorders at the VUB - Brussels Belgium 25
Kuliev Anver Preimplantation diagnosis as part of pre-pregnancy genetic screening USA 25
Lunch 13:20 - 14:00
Session D4: PRENATAL DIAGNOSIS (chairpersons: T. Liehr, E. Schwinger) 14:00 - 15:20
Michalová Kyra Analysis of structural chromosomal aberrations by mFISH and mBAND Czech Republic 15
Liehr Thomas Centromere specific multicolor FISH (cenM-FISH)-a new approach for the characterization of marker chromosomes Germany 20
Karamanov Sultan Combination of optimized PRINS and Spectral MFISH protocols - cheap and fast detection of chromosomal rearrangements in prenatal diagnosis Czech Republic 10
Hořínek A. Prenatal detection of common aneuploidies and fetal sex by quantitative fluorescent PCR Czech Republic 10
Macek Milan Quantitative fluorescent PCR for aneuploidy and mutation detection in microquantity of fetal cells Czech Republic 10
Plesch Andreas Metafer - automated scanning system for rare cell detection and high throughput interphase FISH analysis Germany 10
Benušiene Egle Familial report of unbalanced X;22 translocation Lithuania 5
Coffee break 15:20 - 15:35

Sunday May 13, 2001
Session E
(in main lecture hall)

FIRST TRIMESTER BIOCHEMICAL AND ULTRASOUND SCREENING

Surname Name The title of abstract Country Min
Chairpersons: R. Maymon, H. Cuckle 15:35
Liao Adplpho "Experience with early ultrasound screening and detection of severe fetal anomalies" UK 15
Hyatt Jon Nuchal translucency in cardiac defects UK 15
Christiansen Michael The biochemistry of first trimester serum screening: The role of PAPP-A and ProMB complexes Denmark 20
Chestkov Valery Isolation of PAPP-A from human retroplacental serum Russia 10
Schaeffer Hans-Jörg Comparison of five commercial PAPP-A assays Germany 10
Tsukerman Gennady Feasibility of aneuploidy testing in fetal DNA from maternal circulation within framework of prenatal maternal serum screening Belarus 10
Maymon Ron Sequential first and second trimester screening tests: Correlation of the markers' levels in normal pregnancies Israel 15
Simandlová Martina Anxiety of mothers participating in maternal second trimester serum screening for Downs' syndrome Czech Republic 5
Petersen Michael A common mutation in the methylene tetrahydrofolate reductase gene is not a risk factor for Down syndrome in a population-based study Greece 10
Cuckle Howard Concurrent, sequential, contingent and non-disclosure screening strategies UK 20
Concluding remarks - Howard Cuckle 17:45 - 18:00
Farewell dinner and party in Žofín restaurant 20:00 – 22:30

Scientific meeting of contractors of EU
COPERNICUS-2 project No. ICA2-CT-2000-10012
"Study of genetic and environmental factors in the pathogenesis of most frequent chromosomal anomalies"
Monday May 14, 2001

Surname Name The title of abstract Country Min
9:00 - 10:30
Pelz Jörg Sampling of clinical genetic data by special questionnaire for evaluation of exogenous factors in patients with aneuploidy with regard to the Chernobyl accident Germany 20
Kutsev Sergei Clinical genetic and cytogenetic analysis of patients with aneuploidies linked to the Chernobyl accident in Russia Russia 20
Zerova Tatiana Clinical genetic and cytogenetic analysis of patients with aneuploidies linked to the Chernobyl accident in Ukraine Ukraine 20
Vorsanova Svetlana Clinical genetic and cytogenetic analysis of patients with aneuploidies linked to the Chernobyl accident and aneuploidy patients from other parts from Russia Russia 20
Coffee break 10:30 - 10:45
Schwinger Eberhard Present state and possibilities for the further development of prefertilization and preimplantation prenatal genetic diagnosis Germany 20
Yurov Yuri The collection of DNA probes for prenatal and preimplatation diagnosis by FISH Russia 20
Liehr Thomas Present state and perspectives of cntromere specific multicolor FISH (cenM-FISH)-a new approach for the PGD and prenatal diagnosis Germany 20
Pellestor Franck Present state and further perspectives of multicolor PRINS for PGD and prenatal diagnosis France 20
Diblík Jan Aneuploidy and morphological changes in sperm cells Czech Republic 20
Coffee break 12:25 - 12:40
Petersen Michael B. Present state and perspectives of new technologies in molecular genetic analysis of parental and meiotic origin of aneuploidies Greece 20
Macek Milan Present state and perspectives of QFPCR in prenatal and postnatal diagnosis, parental and meiotic origin of aneuploidy Czech Republic 20
Panel discussion to prepare research plan for years 2001 - 2002 13:20 - 13:50
Lunch 13:50 - 14:30

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